A new method for the detection of Chlamydia trachomatis infection and the serovar determination using polymerase chain reaction and dot-blot hybridization
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منابع مشابه
Detection of Chlamydia trachomatis Using polymerase chain reaction (PCR)
Chlamydia trachomatis now is one of the most prevalent bacteria sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. Diagnosis of Chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. The aim of this study was the detection of Chlamydia trachomatis, using polymerase chain reaction (PCR), beca...
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Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Many species of mycoplasmas are important pathogens that cause respiratory infection in laboratory animals and that are known to affect experimental results obtained with contaminated animals. The aim of the present study was to develop a sensitive and specific assay for the detection of mycoplasma species. To th...
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15 صفحه اولImmune dot blot technique for diagnosing infection with Chlamydia trachomatis.
An immune dot blot test (IDBT) to detect the genus specific lipopolysaccharide chlamydial antigen is described, in which the antigen is trapped on nitrocellulose membrane and then detected with a monoclonal antibody labelled with 125iodine. A preliminary comparison of 270 specimens obtained from the endocervix or male urethra showed that the IDBT was more sensitive (sensitivity 90%) than a comm...
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ژورنال
عنوان ژورنال: Juntendo Medical Journal
سال: 1991
ISSN: 0022-6769,2188-2134
DOI: 10.14789/pjmj.37.416